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The IQ-motif is an amphipathic, often positively charged, α-helical, calmodulin binding sequence found in a number of eukaryote signalling, transport and cytoskeletal proteins.
As expected, there was a significant loss of STAT3 binding to the STAT3 binding sequence found in the survivin promoter in the OSA8 and SJSA lines following incubation with LLL3 when compared to cells treated with media or DMSO (Fig. 4B).
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First, we made a list of high-quality PWMs for factors of known importance in mesoderm development, using sequences from PWMs, using the Flyreg database of DNAse I footprints [32], binding sequences found in recent bacteria-one-hybrid system (B1H) studies [33], [43], and weight matrices from the literature.
The two most optimal binding sequences found in Boger et al. were ATATT and AATAT.
Increased TRAIL gene transcription has been reported in responce to several cytokines and in some cases have been ascribed to transcription factors (Fig. 3) with consensus binding sequences found in the TRAIL gene promoter (Fig. 4).
The amino-terminal copper and nickel binding (ATCUN) motif is a short peptide sequence found in human serum albumin and other proteins.
PTHrP contains in its N-terminal part a consensus sequence found in numerous RNA binding proteins.
The PS binding motif of CagA has been mapped to a consensus sequence found in pleckstrin homology (PH) domains that is responsible for specific phospholipid binding.
Interestingly, the unique sequence found in promoter P1 is analogous to the p53-binding sequence observed in the promoter of another CSC marker, CD44 (Supplementary Figure 3b).
However, we did not consider any possible type-I AdpA binding in the analyses described above; only single AdpA-binding consensus-like sequences found in each AdpA-binding region were used for the analyses (see Discussion).
Additionally, it was confirmed by ELISA that the purified antibodies showed little or no binding to the sequences found in Scramble 1 or 2, or OMPC.
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