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This combined motif was similar to the binding sequence for all significant Spi-B peaks, but the IRF element was more conserved.
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The yeast study with TF binding sites was not confounded by some of the factors mentioned above, since most of the regulatory sequences were experimentally determined, and a comprehensive set of DNA binding sequences for almost all of the yeast TFs were available [ 67].
This region also contains an inverted repeat sequence, which is the binding sequence for TraJ.
Therefore the energy matrix of Figure 1B allows us to calculate the change in binding energy, relative to the consensus sequence, for all possible 7-long sequences.
Motif scanning for the presence of ISRE (Interferon-Stimulated Response Elements), GAS (Gamma-Activated Sites) and NF-kB (Nuclear Factor-kappa B) consensus binding sequences was performed for all 2 kb upstream promoter regions for genes affected by siRNA activity (see Methods).
The program GOMER was used to calculate the Leu3 binding potential for all sequences within 600 bp upstream of an ORF, and to compare the potential of genes in an input list (e.g., bound and regulated genes) to other genes in the genome [ 8].
The comparison of the respective consensus sequences for all these binding sites in the Anastrepha species, C. capitata and B. oleae, revealed a high degree of similarity among the tephritids and Drosophila (Fig. 5).
The two profiles of Pho boxes constructed above were used to scan the genome sequences for all possible SphR binding sites using an algorithm that we developed previously [ 13], which is briefly described as follows with some modifications.
Within the remaining sequences we found binding sites for all twelve transcription factors except PNR and RXRγ.
No binding was observed for all analyzed sequences (data not shown).
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