Thus the task to identify the binding sensitivity of a TF cannot be technically resolved yet by current ChIP-seq, compared to former experimental techniques.
In contrast, the presence of Tyr at this position appears to be important for the high binding sensitivity of clone POR B11, when compared to clones POR A7 and POR D11 that contained a H58 Phe (Fig. 3b).
Taste tests comparing bitter taste perception between humans with different TAS2R38 genotypes combined with biochemical studies have shown that one or more amino acid replacements can alter the ligand-binding sensitivity of TAS2Rs (Bufe et al. 2005).
Both approaches were used in this study to obtain an approximate measure of ligand binding sensitivity and the relative limits of plasminogen binding capacity.
The reversibility, binding stoichiometry, binding affinity, and pH sensitivity of the sensor were studied.
In both cases, burial of the Cl atom within the binding site could explain the observed sensitivity of the binding affinity to Cl deletion (Table 2).
The proposed sub-micron-sized latex particles improved the DNA probe binding capacity, and the sensitivity of the DNA biosensor was enhanced with the incorporation of the highly conductive gold nanoparticles (AuNPs).
Patch clamp recording measures membrane conductance instantaneously, while detection of Annexin-V fluorescence requires the accumulation of Annexin-V on the membrane that is limited by its binding kinetics and the sensitivity of the fluorescence detection.
capillosus ATCC 29799, B. coprosuis DSM 18011, B. finegoldii DSM 17565, B. fragilis YCH46, B. helcogenes P 36-108, and B. salanitronis DSM 18170), because they skew the training set of TF-binding sites and thus decrease the sensitivity of the TF-binding site (TFBS) recognition rule.
The high-affinity (first) site of the trimer displays a dissociation constant of ∼0.01 mM; for sites 2 and 3, one can only estimate binding affinity, which must be at least 1 order of magnitude higher, due to the sensitivity of the binding assay (18, 19).
