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The histone binding selectivity of purified recombinant yeast bromodomains was assessed by using the native core histones in an overlay assay, as well as N-terminally biotinylated lysine-acetylated histone peptides spotted on streptavidin-coated nitrocellulose membrane in a dot blot assay.
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Receptor binding selectivity of radioligand candidate compounds.
Comparing the theoretical binding selectivity of DB18C6 with the experimental one, we verify the feasibility of calculations using the continuum solvation model in predicting the binding selectivity of crown ethers.
The solvation energy of M+-DB18C6 in aqueous solution plays a key role in determining the binding selectivity of DB18C6.
Histone binding selectivity of the yeast BRDs was assayed by a dot blot assay.
Therefore, we hypothesize that binding selectivity of MMA III) will be different from that of arsenite.
Additionally, we characterized the binding selectivities of these proteins toward SGTP and GTP.
CoMPP binding profiles of purified CBM and SusD-like proteins towards a range of cell wall extracts and commercial polysaccharides.
Membrane binding of purified proteins showed that Endo-K8 had similar binding to Endo-WT.
RanBP3 alters the cargo-binding selectivity of CRM1, favouring the binding of NES-bearing peptides over that of Spn1 [23].
Conceptually, the drug design task is more complex and involves appropriate tuning of binding selectivity (avoidance of undesirable targets).
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