Sentence examples for binding selection from inspiring English sources

As an in vitro method, the conditions of the binding selection can be tightly controlled.

Furthermore, Cry1Ac or Cyt1Aa displayed on M13 phages or ribosomes were specifically selected from a mixture of both toxins depending on which antigen was immobilized for binding selection.

Variations of the aptamer selection strategies can be used to isolate catalytic nucleic acid sequences by changing the binding selection step of the aptamer selection process to an activity selection step (Fig. 15).

Thus, the existence of the two natural classes is not due to a simple binding selection.

In this study, we identified a thiophosphate modified aptamer (thioaptamer, TA) against E-selectin (ESTA-1) by employing a two-step selection strategy: a recombinant protein-based TA binding selection from a combinatorial library followed by a cell-based TA binding selection using E-selectin expressing human microvascular endothelial cells.

For the binding selection, random 16mer nucleotide sequences were synthesized (IDT DNA, Coralville, IA) within a 66bp oligo, then made double-stranded by a single round of PCR using primers against the flanking sequences.

We have analyzed the existing database of PDZ domain structures in the context of a specificity tree based on binding specificities defined by peptide-phage binding selections.

Following binding selections to isolate functional clones, DNA sequencing was used to determine the wild-type/mutant ratios at each varied position, and these ratios were used to assess the contributions of each side-chain to antigen binding.

The resulting library was validated by conducting binding selections against a panel of 40 diverse protein antigens and was found to be as functional as a highly validated synthetic antibody library, yielding binders against 30 of the antigens.

Our in vitro binding-selection combined with a powerful display bias normalization revealed characteristic sequence features, but we have to keep in mind that sequence patterns obtained this way are the results of a thermodynamics-driven binding-selection as opposed to a complex natural selection.

Protein 18-19 was the highest affinity sequence from the binding optimized selection and the parent sequence for the current selection.