Sentence examples for binding sections were reacted from inspiring English sources

After quenching endogenous peroxidase activity and blocking non-specific binding, sections were reacted with the primary antibodies listed in Table 1 at 4°C overnight.

After washing step, sections were reacted with 3,3′-diaminobenzidine (DAB) for 10 min and counter-stained with Mayer's hematoxylin.

For immunohistochemistry, paraffin sections were reacted with rabbit anti-periostin antibody or anti-Ki67 antibody (YLEM, Rome, Italy) or anti-fibronectin antibody (Sigma-Aldrich, St Louis, MO) after the endogenous peroxidase had been blocked with 3% H2O2, and nonspecific binding was prevented by treatment with 2% bovine serum albumin (BSA; Nacalai Tesque, Kyoto, Japan) in Tris-buffered saline.

After three rinses in PBS, sections were reacted with secondary antibodies conjugated with RPE for 1 hr at ambient temperature.

Following several washes, the sections were reacted in a 3,3-diaminobenzidine tetrahydrochloride solution (0.07% DAB and 0.002% H2O2).

The 40  μm thick sections were reacted as free floating sections and the 18  μm thick sections were reacted on the glass slides in a humid chamber.

Paraffin sections were reacted with mouse anti-NeuN (1/100) (Chemicon, MAB377).

After washing, sections were reacted with secondary antibodies (Table 1) for 30 minutes at room temperature.

Then the sections were reacted with monoclonal antibody to malondialdehyde (clone 1F83) overnight at 4°C.

Parvalbumin sections were reacted by standard procedures, as described in Ichinohe and Rockland (2005).

Some brain sections were reacted with only mouse anti-GS IgG (diluted 1 : 100; Millipore).