Sentence examples for binding sections were incubated from inspiring English sources

To block nonspecific binding, sections were incubated at room temperature with SuperBlock blocking buffer (Pierce, catalog #37517) for 4 hours.

To block nonspecific binding, sections were incubated with normal goat serum diluted 1∶50 in 5% bovine serum albumin dissolved in Tris-buffered saline (BSA/TBS).

To block nonspecific binding, sections were incubated in 3% BSA for 30 min at room temperature.

To block nonspecific binding, sections were incubated with 2% bovine serum albumin and 2% mouse serum in PBS.

To block non-specific binding, sections were incubated in normal goat serum (Dako) for 20 min at room temperature.

After antigen retrieval, quenching of endogenous peroxidase and blocking of non-specific binding, sections were incubated with primary antibodies (Boster, Wuhan, China) overnight at 4 °C.

To rule out nonspecific binding, frozen sections were incubated in 1 μM random sequences for 5 min. Then, SYL3C-Cy3 was diluted in 250 nM binding buffer and incubated with the sections for 20 min before incubation with DAPI for 3 min to clearly show the position of the nucleus.

For determining nonspecific binding, adjacent sections were incubated in the additional presence of 2.5 μM cold ligand 7A.

To block nonspecific binding, tissue sections were incubated with normal serum appropriate to the secondary antibody species diluted in 0.5% Triton X-100/PBS.

In order to detect primary antibody binding sites, sections were incubated with biotinylated anti-rat (CD31, Ki67, CD41) or anti-rabbit (cleaved caspase-3) antibody for 30 minutes diluted 1∶300 in blocking buffer.

To determine the level of non-specific binding, some sections were incubated with 1 µM of unlabelled allatostatin.