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After considering the influence of the bolt binding coefficient, the overall bending of the binding section and the overall instability of the flange cover plate, the design theory of the earthquake-resilient PSCWJ is established.
Interestingly, the third greatest number of the binding section fell into ion-binding (17%).
Aflibercept is a chimeric protein consisted of the extracellular portion of the human VEGF receptors 1 and 2 (binding section) and the Fc portion of IgG1 immunoglobulin [ 30].
The distribution of the sequences between specialized terms in the binding section of the molecular function category showed that the greatest numbers fell under protein-binding (35%) and nucleotide-binding (19%).
Although the hydrides may well exhibit fluxionality at ambient temperature, their ability to adopt a configuration with a common vertex is required by the reductive elimination (re) mechanism of reversible H2 release upon N2 binding (section 7), and Fe6 is favored from earlier kinetic studies on MoFe protein variants.
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To block nonspecific binding, sections were incubated at room temperature with SuperBlock blocking buffer (Pierce, catalog #37517) for 4 hours.
In order to prevent nonspecific binding, sections were pre-blocked with 1% BSA in PBS for 30 minutes at room temperature (RT).
After quenching endogenous peroxidase activity and blocking non-specific binding, sections were reacted with the primary antibodies listed in Table 1 at 4°C overnight.
To block non specific protein binding, sections were then treated with 5% normal goat serum in PBS-Tween 0.1% (PBS-T) for 60 min at room temperature.
To block nonspecific binding, sections were incubated with normal goat serum diluted 1∶50 in 5% bovine serum albumin dissolved in Tris-buffered saline (BSA/TBS).
To block nonspecific binding, sections were incubated in 3% BSA for 30 min at room temperature.
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