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FREX is demonstrated using fibronectin III, a monobody binding scaffold that has been tailored to recognize multiple targets.
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That is due to level 0 being the root level which contains only a single scaffold, the root scaffold, that has exactly 102 children (distinct ring count scaffolds).
Three-helix bundles and coiled-coil motifs are well-established de novo designed scaffolds that have been investigated for their metal-binding and catalytic properties.
As we mentioned above, CS scaffolds possess distinct bioactivity and biopolymer-modified CS scaffolds that have excellent mechanical strength [ 11].
We focused on scaffolds that had at least 20 SNPs per scaffold to avoid bias from falsely called SNPs.
The putative tumor metastasis suppressor 1 MTSS1) is an actin-binding scaffold protein that has been implicated to play an important role in carcinogenesis and cancer metastasis, yet its role in the development of gastric cancer has not been well illustrated.
Several binding scaffolds that are not based on immunoglobulins have been designed as alternatives to traditional monoclonal antibodies.
The two closely related binding modes that have been proposed for the published class of benzimidazole CHK2 inhibitors both postulate that a pendant carboxamido substituent on the benzimidazole scaffold interacts directly with the hinge (Fig. 1).
Afadin is a scaffold protein containing an actin-binding and Ras/Rap-binding domain [ 29, 49] that has been reported to regulate the cyclical activation/inactivation of Rap1 and RhoA [ 50].
Additional functions (inhibition of oxidation and protonation of cysteine) of the phenylalanine and heme binding motif can be inferred from studies that have introduced a thiolate into a different proximal surface scaffold.
"These scaffolds are mechanically the softest electronic materials that have ever been made," says Lieber.
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