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A large panel of serotype specific phage expressing botulinum binding scFv could be selected from both libraries.
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The fact that a single monoclonal, monovalent cross-reactive scFv could be found with binding affinity against both cholesterol and MA corroborates the conclusion reported by Benadie et al. (2008) that cholesterol and MA share some antigenic structural properties and may explain why TB negative sera recognize cholesterol as well as they do MA.
Functional studies showed that the A21 scFv could be internalized with high efficiency after binding to the ErbB2-overexpressing cells, suggesting this regent may prove especially useful for ErbB2-targeted immunotherapy.
BIAcore assays show that SC142 scFv has an ideal off-rate constant and SC142 scFv could be a good tumour imaging reagent.
In this regard, we wondered whether the observed inhibition of cell proliferation by the scFvs could be due to competition with pRb for binding to E7.
In particular, functional scFvs could be expressed.
Of the 50 isolated clones, only 24 full-sized sequences coding for scFvs could be obtained.
ScFvs with different binding specificity could be employed either independently, to discriminate the different functions of E7 thus gaining an insight into the mechanism of E7-mediated deregulation of cell proliferation, or in combination, to enhance their anti-E7 activity.
This different binding activity could be ascribed to the oligopeptide growth proceeding from COOH- to NH2-terminus, with the COOH- terminus bound to the rod possibly resulting in less accessibility for the physical interaction with the scFvs.
This strong binding could be confirmed by localizing CD9 staining on MKN-28 with the scFv-Fcs using confocal microscopy.
This binding could be inhibited by heparin.
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