Sentence examples for binding samples were incubated from inspiring English sources
To determine specificity of DNA binding, samples were incubated with or without 20 ng of unlabeled competitor DNA for 10 min at room temperature.
For protein-DNA binding, samples were incubated with 200 μg of poly(deoxyinosinic-deoxycytidylic) acid (Sigma) for 15 minutes at 4°C, then incubated with the biotinylated oligonucleotides for 2 hours at 4°C.
For the binding reaction, samples were incubated at 30°C for 10 min or the times indicated.
To eliminate nonspecific protein binding, the samples were incubated with 10% normal goat serum for 30 min at room temperature.
Then pairs of filtration tubes were incubated either with a 1000X cold FA in a binding solution (10 mM Na2P4, 1.8 mM KH2PO4, 500 mM NaCl, 2.7 mM KCl, pH 7.4, and 25 mM n-octyl-β-D-glucopyranoside) or with the binding solution alone, and samples were incubated 2 hours at room temperature and then centrifuged.
To block nonspecific binding of the antibodies, the samples were incubated with 1 % bovine serum albumin (BSA) in PBST for 1 h and washed twice with PBST, with each wash lasting 5 min.
L19-SIP (125 ng), labelled with I or I, was added to the tubes and the samples were incubated overnight at 4°C for binding.
After addition of chaotropic lysis buffer, the samples were incubated for 15 min at 58°C, followed by incubation with DNA binding beads for 15 min.
The samples were incubated for 10 120 minutes to allow binding, then loaded on a 5% polyacrylamide gel, and run at 10 Watts.
Where indicated (Figure 4), after binding the virus to erythrocytes or PHA-stimulated PBMC at 4°C, samples were incubated at either 4°C or 37°C for 4 hours.
