The biotin binding residue in human ACC2 has not been clearly identified, yet structural studies using nuclear magnetic resonance suggests that biotin is attached to lysine 929 within a BCCP domain (Lee et al. 2008).
Putative binding residues in the dataset TS75 were then predicted.
We note that while the core effector-binding region of CED-10 is 100% conserved with human Rac1, Rac2 and Rac3 (YIPTVFDNY), RAC-2 has nonconservative variants in two residues (YI LTVFD TY), and mutation of many of these residues in human Rac1 disrupt binding of Pak and other effectors (Westwick et al. 1997).
Random mutagenesis and automated screening in yeast identified residues in human PP2Acα important for inhibitory FOS binding.
Without exception, the affected residues in the Ts– strains lie within the dimerization interface encompassing the GTP-binding pocket (the G interface), based on the locations of the equivalent (and highly conserved) residues in human septins.
Remarkably, three of these correspond to residues in human Sds22 (D148, E192, F214; boxed residues in Figure 2B) that are required for proper PP1 binding (Ceulemans et al. 2002).
The presence of insecticide residues in human milk has been established by Food and Drug Administration scientists.
Thus, all of the residues in human FKBP12 required to interact with Tor are sufficiently conserved indicating that the human FKBP12-rapamycin complex is fully proficient in binding and inhibiting the yeast Tor1 and Tor2 proteins.
The extracellular regions of DDRs consist of an N-terminal DS domain that contains the collagen-binding site (~ 160 residues) [34,35], followed by a recently described DS-like domain (~ 180 residues) [36], and an extracellular JM region that is poorly conserved between DDR1 and DDR2 (50 residues in human DDR1, 32 residues in human DDR2) (Fig. 1).
The residue corresponding to T431 in vSGLT (T460 in human SGLT1) was even suggested to be a substrate-binding residue in SGLT1 because its mutation to cysteine altered sugar selectivity and decreased the affinity for glucose.
