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Binding was quantified by phosphorimaging and is expressed as the level of binding relative to the wild-type protein.
Two glycopeptide chimeras corresponding to the Shigella flexneri Y O-polysaccharide and its peptide mimic were designed in an attempt to improve the binding affinity by increasing the entropy of binding relative to the original octapeptide mimic of the O-polysaccharide.
Their results showed that all variants had similar potency in PD assays and rat FcRn binding relative to the starting material.
At first, a set of noiseless TACs (n = 10) was generated to assess whether model preference was associated to the level of specific binding relative to the non-specific signal present in that particular region.
Second row: SUV image (60 90 min p.i .. Third and bottom row: DVR (from dynamic data reconstructed with 10-mm Gauss filter) and SUVR with lower threshold set to 1.0 for the depiction of specific binding relative to the PRR. a Whole head in triangular views.
The Rmax was then calculated, and the binding relative to the Rmax from three independent experiments was averaged.
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For native HLA, at least, membrane curvature is of secondary importance for binding relative to membrane charge and the protein's membrane-bound folding state [24], [28], [29].
Figure 2 now quantifies AnnexinV binding relative to ANO6 expression and shows the time course of AnnexinV binding.
Control studies on AMP binding relative to ATP are also shown in the Supporting Information, wherein similar binding affinities are observed to be consistent with literature results.
In comparison, the ΔΔ C p for NW binding relative to glutamate is more negative (−671 cal mol 1 K–1), suggesting that the Δ C p for NW binding relative to that of the apo form would be considerably more negative than for glutamate binding.
Coupling the structural analysis with mutagenesis studies of two other malonyltransferases from the same species also revealed a greater structural diversity of acyl acceptor binding sites relative to the acyl-CoA donor binding sites [ 7].
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