Even in degenerate discs, however, the disc cells can retain the ability to synthesize large aggrecan molecules, with intact hyaluronan-binding regions, which have the potential to form aggregates [ 24].
Promoter sequence regions which have been annotated as known transcriptional factor binding sites were masked out.
According to us, these two regions, which have 10 nucleotides after the potential transcriptional initiation base, could contain binding site(s) for transcription factor(s).
This study determined whether neuronal populations in the rat olfactory bulb and hippocampus (brain regions which have very high concentrations of insulin receptors) also express IRS-1 and contain phosphotyrosine, using in situ hybridization, receptor binding, and immunocytochemistry.
HIV-1 viral envelope regions, which have been identified to be immunogenic and to which mAbs were developed include the V1V2, V3, CD4-binding domain (CD4bd), CD4 induced antigen (CD4i) and C5 regions of the gp120 [9].
ChIP DNA was subject to PCR using specific primers flanking a piece of DNA sequence of MKP7 promoter region, which has binding sites for RGS2 or HIF1 α.
Each of these proteins had large binding regions which offered ample room for the ligand to bind in multiple conformations and at multiple sites within the binding region of the protein.
The Ascl1 B site is located within the Ascl1 promoter region, which has been characterized previously and contains 3 additional, non-conserved Gli binding sites [55].
This seems reasonable, as FN has three Hep binding regions, which also might be electrostatically favorable for the interaction with sHA1 but not with non-sulfated HA.
