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In the previous procedure, the transcription factor binding regions were set as the promoter regions [ 24].
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ChIP-seq binding regions overlapped by ChIP-chip binding regions were used for downstream analysis.
Implant region was set as region of interest (ROI).
Implant region was set as ROI.
Positive and negative control binding regions were established using different primers sets, and data was expressed as percentage of input for each region.
CRMs and Singleton binding regions were classified in accordance to their conservation status, and these separated sets were used as input.
Transcription factor (TF -binding regions were exTF -bindingng the filteregionslatory_feature_type_name" set to "TF binding site".
By manual inspection, the binding region was checked for the orientation of Ensembl known transcripts relative to the probe set.
(C) Binding activity of EZH2 to the C/EBPβ binding region was analyzed by ChIP assay.
This binding region is not similar to those previously identified as Rac binding motifs.
The NADPH binding region is yet unclear.
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