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DNA sequences for LRH-1 binding regions were retrieved using Galaxy (http://main.g2.bx.psu.edu) and used for motif search using MEME [ 16].
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The SNP site flanking regions were retrieved from NCBI SNP reference assembly database (Build 131).
Genes that reside in the identified CNA regions were retrieved using SQL queries according to their genomic locations.
Complete lists of human EntrezGenes included in these regions were retrieved (Additional file 6).
Flanking DNA sequences within 10 kb upstream and downstream of the matched regions were retrieved.
SNP data for the human genomic regions was retrieved from dbSNP, build 114.
Finally, measured soil moisture data from a semiarid region were retrieved from the Automated Weather Data Network.
Sea surface temperatures for the region were retrieved from the NCEP-NCAR database.
The chromosomal coordinates for each linkage region were retrieved using UCSC genome browser.
From the resulting 50 unique genes the promoter region was retrieved, which was used for enrichment analysis of transcription factor binding sites (TFBS).
The genomic sequence of murine Nrf2 containing the promoter region was retrieved from NCBI mouse genome data base.
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