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Based on the expression profile of the nearest gene, the binding regions were grouped into those associated with PPARγ-regulated and non-regulated genes.
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The genes with E-box binding region was mostly clustered within the subfamilies 1 5, 12 14, and 16 20, whereas the genes with non-DNA-binding region were grouped in the subfamilies 6 11 and 21.
Groups of binding regions were classified as ECC only, Limb only, PNST only, ECC-Limb, ECC-PNST, Limb-PNST, or ECC-Limb-PNST.
208 binding regions were annotated for these 19 factors.
ChIP-seq binding regions overlapped by ChIP-chip binding regions were used for downstream analysis.
Transcription factor (TF -binding regions were exTF -bindingng the filteregionslatory_feature_type_name" set to "TF binding site".
Since the intron insertion site, which was supposed to be in the G-protein beta binding region, was exclusively conserved in the group of GIRK genes, we attempted to analyze the genomic structures of G-protein betas interacting with these GIRKs.
(C) Binding activity of EZH2 to the C/EBPβ binding region was analyzed by ChIP assay.
This binding region is not similar to those previously identified as Rac binding motifs.
The binding region was detected using primers in PCR reactions.
The binding region was detected in PCR reactions.
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