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The top 50 EIN3 binding regions were determined and the repeatmasked.
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The transmembrane regions are determined by TMHMM [17] and the glutamate binding regions are determined by sequence alignment.
Once the binding region is determined, we obtain the binding path on it by docking an arbitrarily chosen peptide using AutoDock [33].
Positions of six LRR repeat regions, ATP binding site, active sites and nucleotide phosphate-binding region were determined by PROSITE and results are depicted in Figure 2.
Both the proportion of genes with RAX-binding motifs and the number of RAX-binding motifs in the 5 kb promoter regions were determined in the deregulated genes and randomly selected genes.
The binding ability of different TFO RNAs (TFO1 to TFO5) against their target regions was determined by EMSA.
A 5kb upstream sequence from the transcription start site of each of these genes was retrieved and the binding sites of transcription factors within this region were determined as described above.
5.0 kb upstream regions from the transcription start sites of such genes were retrieved using Ensembl [34] and the transcription factor(s) binding within this region was determined using Over-represented Transcription Factor Binding Site Prediction (OTFBS, Release 6.0).
For the rest of the X chromosome, once the DCC binding regions were excluded, we determined an average of 602 ± 24 sites/Mb, a value almost identical to the one that we found for the autosomes: 590 ± 29 sites/Mb (all large autosomal arms considered).
In a CLIP-pyrosequencing experiment, the resolutions of the detected protein binding regions are partially determined by the length of the detected RNA fragments (CLIP amplicons) after trimming by RNase digestion.
MYC binding to the miR-146a promoter and a negative control region was determined using the primers listed in Supplementary file 1E.
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