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We also identified the MHC class 2 binding regions using the ProPred-2 online service [26].
We identified three novel AR matrices (Figure 4) that can be mapped to 1,448, 1,012, 317 AR binding regions respectively (Table S6 S7), which is much higher than the number of mapped AR binding regions using the three known canonical AR motifs from the Transfac database as we showed above.
We report an empirical approach to improve the resolution of binding regions using the likelihood of RNase digestion (estimated in Eq. 1) within local genomic regions.
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Our approach to identify the TF-binding regions was to first determine the binding locations from each dataset, and then combine the binding locations from at least five of six datasets to define a binding region, using the recently developed MetaScope visualization software and genome browser [ 27].
Each log-ratio dataset (from duplicate or triplicate samples) was used to identify TF-binding regions using the software (width of sliding window = 300 bp).
Previously, two similar high-throughput methods were developed for the identification of protein binding regions using a large population of fragments derived from DNAs (plasmids, bacteriophages, bacterial chromosome and human genome fragment) ranging in size from 3 kb to 4,700 kb [ 10, 11].
ANCHOR aims to capture the basic biophysical properties of disordered binding regions using estimated energy calculations (Mészáros et al., 2009).
The 250-bp region was analyzed for potential transcriptional binding regions using Genematix platform (Munich, Germany).
Bolton et al. designed tiling arrays around the transcription start sites of 548 candidate hormone-responsive genes and they identified 524 AR binding regions using ChIP to chip analysis [34].
A complementary approach identifies protein binding regions using motif searches.
Attempts have been made to find such binding regions using purely computational approaches.
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