We previously reported that a novel dimer peptide (apoEdp) derived from the receptor binding region of human apolipoprotein E (apoE) inhibits virus-induced angiogenesis.
Previously, residues within the transmembrane region that are involved in the binding region of human CCR4 have been identified by mutational analysis; they include: Leu 42 and Tyr 46 (Trp), Trp 45 (TM2), Ile 113, Tyr 117 and Phe 121 (TM3), Ser 203 and Ile 206 (Tyr), Tyr 258, Asn 259 and Leu 262 (TM6) and Glu 290 (TM7).
Thus, there is about 71% identity and more than 91% similarity in the RPA70-binding regions of human and hydra XPA proteins, indicating a high probability that XPA is able to bind to its putative RPA70 counterpart in hydra.
No reports are available on whether a HSPG-binding peptide derived from the receptor-binding region of human apoE E can inhibit tumor growth by blocking angiogenesis.
Specific peptide sequences derived from the receptor-binding region of human apoE have been previously reported by us [19], [20] and others [17], [18], [40], [41] to have anti-infective and anti-inflammatory properties.
We found that the B. burgdorferi Pur-α and the nucleic acid-binding region of human Pur-α bound with comparable affinities to DNA oligomers with (GGN n sequences (Table 1).
Finally, Xrc4 physically interacts with Lig4 through a Lig4 region homologous to the XRCC4-binding region of human LigIV.
Even within the Ca2+-binding region of human TCTP, which consists of a stretch of 30 amino acids, only three out of 6 amino acid residues are conserved between human and plant TCTPs.
The zebrafish full-length Apoc2 (NCBI Gene ID: 568972, molecular mass 11.2 kDa) is only 27.8% identical to human APOC2, with 49.5% conserved consensus amino acids, but its C-terminal region (residues 67-92, zebrafish numbering), which mediates Apoc2 binding to Lpl, shows 46.1% identity to the LPL-binding region of human APOC2.
To study the association of CaM with a biotinylated peptide corresponding to the CaM-binding region of human eEF2K (residues 76 95), or a variant which contains phosphoserine, not serine, at position 78, the wells of a Maxisorp ELISA tray (Nunc) were coated overnight at 4°C with CaM (0.2 μg/ml) in PBS.
