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The location of binding regions, defined by their summit region coordinates relative to RefSeq gene structure, was determined using CEAS version 0.9.9.8 (Shin et al., 2009; http://liulab.dfci.harvard.edu/CEAS/).edu/CEAS/
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We performed antibody labeling to localize the membrane-proximal region of the receptor (using a monoclonal antibody against the C-terminal Flag tag) and the ligand-binding region using a monoclonal antibody raised against EGF repeats 12 20 of the Drosophila Notch receptor that encompasses at least part of the ligand-binding region defined by EGF repeats 11 13 [8], [12] (Figure 3A, Figure S5).
Prokaryotic transcription initiates in promoter DNA regions, defined by the presence of binding sites for a dissociable RNA polymerase subunit called sigma factor.
One of four geographical regions defined by the United States Census Bureau.
(A ) Boxplots demonstrating the distribution of mean ChIP enrichments for enhancer regions defined by H3K4me1 and H3K27ac marks in ESCs (see Creyghton et al., 2010 for details) that overlap with the clusters of binding defined by our ChIP-seq samples.
We further validated the binding of Myc to genomic loci proximal to miR-141, miR-200, miR-338, and miR429 by qChIP PCR using primers to amplify the bound regions defined by our array analysis.
SNPs rs662694 and rs471467 localise to a predicted insulator region, defined by CTCF binding and H3K27me3 marks (44).
In a AAA+ ATPase, the nucleotide binding pocket is the most sequence-conserved region, defined by the essential Walker A and B motifs.
We also have added to the diagram in Figure 1A the region defined by Reinhardt et al. (2007) as sufficient to bind CK1 (it overlaps with the PP2A binding region).
Previous mutagenesis study of Ca2+-coordinating residues suggested that the loop region defined by Glu99 and Asp114 might be the ice-binding site [23].
For a particular region defined by the user, it is possible to explore the list of CBS predictions of binding sites that are located within potential enhancers in the vicinity of the gene of interest.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com