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There are two major functional domains, the N-terminal domain (NT), contains the major receptor binding region, the C-terminal domain (CT), contains the lipid binding region, which is also thought to bind Aβ [9], [10].
Experimental studies on apoE are focused on its receptor binding region, which is located between residues 130 150 and is critical for its biological activity.
The signal molecule binding region, which is located in the N-terminal portion of the protein and a helix-turn-helix motif (HTH) located in the C-terminal, which is responsible for the protein binding to the target promoters [13] [15].
It consists of an extracellular ligand binding region, which is connected to the cytosolic region through a hydrophobic transmembrane domain.
Interestingly, A. niger P43 has GST C-terminal domain at the N-terminal of the tRNA binding region, which is different from the human counterpart.
Immediately adjacent to the N-terminal J-domain of Class I J-proteins is a glycine/phenylalanine (GF -rich linker reGF -richlinker by the client pregion binding region, which is composed ofollowedrrel topology domains, CTD1 and CTD2.
Similar(53)
The docking site of MX2 was identified by the nucleotide binding region which was recorded as the relation with GTP function.
The mutations of these residues within the Mdm2/MdmX binding region, which are normally phosphorylated downstream of DNA damage, result in constitutive p53 activation.
GenBank comparison (BLAST) showed that the protein was closely related to P450 because a heme-binding region, which is highly conserved in all P450 sequences, was found in the ORF protein.
As also described previously [J. Biochem. 130 (2001) 657], pol β and topo II have a three-dimensionally similar triterpene-binding region, which is a pocket in which specific compounds can insert.
For the second nested PCR of CYP genes, a degenerate forward primer was constructed based on the relatively conserved consensus aa sequence between the CYP O2-binding region and the CYP heme-binding region, which is called a meander region (Hasemann et al. 1995), as shown in Table 1.
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