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This binding region is not similar to those previously identified as Rac binding motifs.
Furthermore, since the RNA binding region of Tat was still intact in this mutant, this experiment also shows that the RNA binding region is not sufficient to release P-TEFb from the 7SK snRNP.
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We show that some cryptic epitopes on gp120 are accessible to the elicited antibodies, and some epitopes in the CD4 binding region are not accessible.
Since RNA binding region was not clearly understood for DDX3X, we carried out a structure based alignment using DDX3X structure as query (in CATH database) and identified DDX4 as a DEAD box helicase which shares >70% similarity with DDX3X.
By contrast, the antibody against EGF repeats 12 20 shows that the ligand-binding region is not exposed on the most membrane-distal surface of the NECD but rather on the side of the receptor (Figure 3B).
However, this model is buried in Figure 3 figure supplement 1F and the ATP-binding region is not highlighted, which makes it hard to evaluate.
Remarkably, four of the deviations detected by mapping located in the general primer binding regions were not detected in the assemblies.
However, RREB1 occupancy at shared binding regions was not detected in ECCs.
Binding regions located in the first intron of Dsp, upstream of Dsc3, upstream of the Dsg1 gene were strongly enriched in ChIP-qPCR with anti-p63 antibodies compared with control antibodies, whereas the other tested binding regions were not enriched (Fig. 5A).
A recent Cameroon study also found that seroreactivity to the CSA-binding region was not associated with gravidity.
Notably, while AidB shares sequence homology and structural similarity with members of the ACAD family such as IVD and medium chain acyl-CoA dehydrogenase (MCAD), the AidB L1′2′ loop and the DNA-binding region are not conserved in any other members of the ACAD family.
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