Thus, our finding that the EB binding region is a stabilizing structural cap suggests that the phenomena of microtubule dynamic instability and +TIP activity are intimately linked.
We next tested whether this GR binding region was a mediator of glucocorticoid response.
ASK1ΔN, which is an N-terminal truncated mutant of ASK1 lacking a Trx binding region, is thus a constitutively active mutant and more potently induces apoptosis in various cultured cells [ 3, 13, 14].
If the binding region is within a gene, CEAS software indicates whether it is a 5′UTR, a 3′UTR, a coding exon or an intron.
As depicted in Additional file 1, the RTK-derived docking oncoproteins consist of an oncogenic form of the Met receptor, Tpr-Met, in which the multi-substrate binding region is replaced with a motif selective for the recruitment of a single signaling protein, found in other RTKs [ 8].
However, we cannot exclude that the Hoxb4 3'UTR binding region is located in a sequence bridging R1 to R2 or R2 to R3.
Hydrophilicity within the cavity (triphosphate binding region) is created by a tetrad Gly181/Ser182/Gly183/Lys184 along with Arg133.
The β2 spectrin N-terminal actin binding region is composed of a tandem calponin homology domain designated CH1-CH2.
Taken together, our results show that the IKKβ binding region is natively ordered as a coiled coil within the NEMO dimer, and identify noncovalent interactions in the region of NEMO centered on Cys54 as a major contributor to coiled-coil stability.
In SA, only one of the two 65-amino-acid repeats of the cell wall binding region is necessary for binding, and an enhancing role for the other repeat has been suggested (Smit and Pouwels 2002).
