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All MEF2 proteins have an N-terminal DNA binding region composed of MADS (Minichromosome maintenance, Agamous, Deficiens, Serum response factor) and MEF2 domains, two central transcription activating domains (TAD1 and TAD2) and a C-terminal nuclear localization sequence (Fig. 1B).
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A secretary signal sequence locates at the N-terminus followed by a ligand-binding A region and a characterized R region composed of SD repeats.
For example, external loops thought to be responsible for phage binding contain many more positively selected sites than the internal β-barrel region (composed of strands).
These proteins belong to a distinct group of MerR-like DNA-binding domains known as truncated HTH domains that lack the Wing 2 region composed of two α-helices and the long dimerization helix [ 52, 53].
Vertebrate filamins are non-covalent dimers of 240 280 kDa subunits composed of an N-terminal actin-binding domain formed from two calponin homology domains followed by a rod region composed of 24 tandem immunoglobulin-like domains (IgFLN1 24) (Gorlin et al, 1990; Stossel et al, 2001; van der Flier and Sonnenberg, 2001).
The master regulation exerted by Sox2 and Oct4 during mammalian embryogenesis is believed to operate through their cooperative binding to DNA regulatory regions composed of adjacent HMG and POU motifs (HMG/POU cassettes) [ 60].
Organisms have a linear genome that contains genes and their upstream regulatory regions composed of transcription factor binding sites (TFBSs).
The β2 spectrin N-terminal actin binding region is composed of a tandem calponin homology domain designated CH1-CH2.
The selectins have characteristic extracellular regions composed of an amino-terminal lectin domain that binds a carbohydrate ligand, an epidermal growth factor-like domain, and two to nine short repeat units homologous to domains found in complement binding proteins.
Unfortunately, none of the polymerases we studied showed significant activity with gNTP substrates and GNA templates, even when the primer and primer-binding region were composed of DNA.
Immediately adjacent to the N-terminal J-domain of Class I J-proteins is a glycine/phenylalanine (GF -rich linker reGF -richlinker by the client pregion binding region, which is composed ofollowedrrel topology domains, CTD1 and CTD2.
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