Sentence examples for binding reactions with the from inspiring English sources
The BIAcore approach is based upon the ability to detect changes in mass on a sensor surface that, when corrected for non-specific interactions between the analyte in solution and unmodified sensor surfaces, reflect binding reactions with the immobilized ligand.
(C ) Binding reactions with the RA104 mutant transposase were set up as in Figure 3B.
Binding reactions with these proteins were carried out as described above.
We developed a strip biosensors array based on aptamer-modified gold nanoparticles as receptors and combined the protein-aptamer binding reaction with the streptavidin-biotin interaction as well as the sandwich format.
In contrast, addition of SpxR60E negatively affected the DNA-αCTD-wild-type Spx complex (Figure 7A, lane 13), and completely abolished the DNA-αCTD binary complex (Figure 7A, lane 14) when it was included in the binding reaction with the G52R protein (compare with Figure 7A, lane 11, a reaction containing the G52R mutant protein alone with αCTD).
To examine kinetic parameters of the dendrimer-induced blockage, we focused on a dendrimer binding reaction with the PA63 channel using current noise power spectrum analysis.
The KD for HSP72 activity control binding reactions was estimated at 1-50 mM based upon published data from the analysis of binding reactions with DnaK, the bacterial analogue of HSP72 (6).
The model incorporates both thermodynamic and kinetic properties of the amphoteric hydrogen ion site-binding reactions with the surface OH groups.
Since the amount of bound Ca2+ is frequently measured in Ca2+-CaM or other ligand-multisite protein interaction experiments, these results allow a direct quantitative comparison to be made of the binding reactions with and without the presence of cooperative binding.
For EMSA, 3 μg of the nuclear extract was utilized for the binding reactions with 20 fmol of biotin-labeled double stranded oligonucleotide probe.
