Sentence examples for binding reactions were performed from inspiring English sources

Binding reactions were performed for 30 min on ice with 50 µg nuclear extract and (γ-32P) ATP labeled oliginucleotide.

Binding reactions were performed in RNA binding buffer (10 mM Tris-HCl (pH 7.4), 10 mM KCl, 1 mM MgCl2).

Binding reactions were performed in the presence or absence or 0.1 mM cap analogs (GpppG or m7GpppG).

DNA-protein binding reactions were performed by incubating 5 µg of nuclear protein with excess 32P-labeled CRE oligonucleotide in the buffer supplied with the assay kit.

The binding reactions were performed using the LightShift Chemiluminescent EMSA Kit (Pierce, Rockford, IL) and conducted according to the manufacturer's protocol.

All binding reactions were performed in 50 mM Tris-HCl pH 7.5,100 mM KCl and 10 mM MgCl2 at room temperature.

In order to understand the immunological detection mechanism of GO and AuNP-GO nanocomposites, spectral analysis for binding reactions was performed as shown in Fig. 6. Figure 6a shows the UV-vis absorption spectra of the AuNP-GO and GO-BSA nanocomposites.

Briefly, DNA protein-binding reactions were performed for 10 minutes at room temperature with 15 µg nuclear extract, 1 µl P32 labeled oligo, and 4 µl of binding buffer containing poly dI/dC.

Cap-binding reactions were performed at 4°C with one mg protein and 15 µl m7GTP-sepharose or sepharose-4B (GE Healthcare) in NT2 buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM MgCl2, 0.05% NP40] for one hour.

The DNA-binding reactions were performed in a volume of l0 µl binding buffer provided by the manufacturer containing poly d(I C) at 15°C.

The DNA-binding reactions were performed using purified glutathione-S-transferase (GST), GST-EWS-Oct-4, or GST-EWS-Oct-4B proteins for 30 min at 4°C in binding buffer containing 10 m M Tris-HCl (pH 8.0), 40 m M KCl, 6% glycerol, 1 m M DTT, 0.05% NP-40, and 10 ng  μl−1 of poly (dI dC) (dI dC).