Sentence examples for binding reactions were conducted from inspiring English sources

Binding reactions were conducted by mixing nucleic acids and proteins in the binding buffer (a commercial resource from Beyotime), in a final volume of 20 µl.

To create a pseudo-first-order condition that simplifies the kinetic analysis, all binding reactions were conducted under conditions of Dtot ≪ Ptot ≪ Ctot.

Binding reactions were conducted in solution at 37°C for 14 16 h in the early and for 1 3 h in the later rounds.

Whenever possible, the binding reactions were conducted at multiple temperatures, so that the change in heat capacity (ΔΔ C p ) of the binding reaction could be determined.

The binding reactions were conducted with 5 μg of nuclear extract and of 2 × 105 cpm of [γ-P]-labeled oligonucleotide probe at 22°C for 20 minutes in a final volume of 10 μl, and complexes were resolved on non-denaturing 6% polyacrylamide gels.

The binding reaction was conducted at room temperature for 20 min with 5 μg of nuclear extract, 40,000 dpm (0.08 0.4 ng) of radiolabeled oligonucleotide probe, in 1x Ficoll buffer (10 mM Tris (pH 7.5), 1 mM DTT, 1 mM EDTA, and 4% Ficoll), 250 ng of poly(deoxyinosinic-deoxycytidylic acid) in 75 mM KCl, and double-distilled H2O to make the volume to 15 μl.

Reactions were conducted for 1, 24, or 72 at 37 °C.

Enzymatic reactions were conducted for 15 min at 37 °C.

Reactions were conducted at 50 °C for 10 min.

Triplicate PCR reactions were conducted.

Assembly reactions were conducted in 3 steps.