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Binding reactions were analyzed by SDS-PAGE using CA tubular assemblies at 80 μmol/L or binding buffer alone, incubated with His-sumo-N-GF and N-GF (79 μmol/L).
Binding reactions were analyzed by electrophoresis in a 4% non-denaturing polyacrylamide gel.
The binding reactions were analyzed on a 5% polyacrylamide gel in 0.5× TBE and quantified using a phosphoimager system.
Binding reactions were analyzed on 6%0.5×× Tris-borate-EDTA (TBE) polyacrylamide gels electrophoresed at 150 V; gels were dried prior to detection of signal by autoradiography.
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The results of the binding reaction were analyzed in 4.5% acrylamide/bisacrylamide gels run at constant 130 V and dried (80°C, 1 h).
Sequencing reactions were analyzed on an Applied Biosystems 3100 DNA analyzer.
The reactions were analyzed by autoradiography.
Binding reactions were set at room temperature in binding buffer (10 mM Na2HPO4 pH 7.2, 60 mM KCL, 1 mM EDTA, 7 mM β-mercaptoethanol, 5% glycerol, and 50 ng/µL of poly-C) in a volume of 10 µL for 20 min. Reactions were analyzed by electrophoresis into a non-denaturing 10% polyacrylamide gel, which was dried and exposed to film overnight.
Reactions were analyzed by the ABI 7900HT (Applied Biosystems).
qPCR reactions were analyzed on BioRad IQ5 (BioRad Laboratories).
The reactions were analyzed as described above.
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