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The protein binding reactions were also performed as previously described (Badis et al. 2009).
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A fraction of the protein in each binding reaction was also loaded on the gel.
These reactions were also incubated in binding buffer with the C2-L1Tc protein (0.67 μM) at 37 °C for 5 min.
Hypersensitivity reactions were also observed.
Binding reactions were scaled up for immunoprecipitation of photoaffinity-labeled subunits.
Binding reactions were the same as in (A).
Binding reactions were set up with varying protein combinations.
For supershift assays, binding reactions were set as described above.
Binding reactions were allowed to take place overnight at 4°C.
Binding reactions were carried out in triplicate.
Binding reactions were incubated for 20 min at room temperature.
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