Sentence examples for binding reaction with the from inspiring English sources

We developed a strip biosensors array based on aptamer-modified gold nanoparticles as receptors and combined the protein-aptamer binding reaction with the streptavidin-biotin interaction as well as the sandwich format.

In contrast, addition of SpxR60E negatively affected the DNA-αCTD-wild-type Spx complex (Figure 7A, lane 13), and completely abolished the DNA-αCTD binary complex (Figure 7A, lane 14) when it was included in the binding reaction with the G52R protein (compare with Figure 7A, lane 11, a reaction containing the G52R mutant protein alone with αCTD).

This result means that Seq15_12_35 can have a tight binding reaction with the Ang2.

To examine kinetic parameters of the dendrimer-induced blockage, we focused on a dendrimer binding reaction with the PA63 channel using current noise power spectrum analysis.

The BIAcore approach is based upon the ability to detect changes in mass on a sensor surface that, when corrected for non-specific interactions between the analyte in solution and unmodified sensor surfaces, reflect binding reactions with the immobilized ligand.

Binding reactions with these proteins were carried out as described above.

To further verify the binding of YY1 to the DNA sequence in the FEN1 promoter region, we added non-specific IgG or anti-YY1 anti-body to the binding reaction with YY1 and WT FEN1 sequence.

The dashed curve in Figure 4 A represents a binding reaction with an equilibrium dissociation constant of 1.1 × 10 9 M, the value for the binding of PSA to antibody M612166 that was reported earlier using surface plasmon resonance measurements.

The rectangular hyperbola drawn through the data points in Figure 3A describes a one-site homogeneous binding reaction with an equilibrium dissociation constant of 5.6 × 10 8 M. Kinetic data for the bimolecular association of PSA with antibody M612165 are shown in Figure 3B.

After centrifugation at 40,000xg for 20 min (Sorvall), supernatants were subjected to a binding reaction with Ni2+-NTA (Qiagen) resin for 30 min in a batch procedure.

ELISA results showed that HRP-LD5 had a much stronger binding reaction with hIgM and hIgA than did HRP-goat anti-human PcAb, and had similar binding to hIgG compared with HRP-goat anti-human PcAb (Fig. 1).