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The specificity of the binding reaction was determined by coincubating duplicate samples with unlabelled probes.
The specificity of the binding reaction was determined by coincubating duplicate samples with either 100-fold molar excess of unlabeled oligonucleotide probe or an anti-NF κB antibody (anti-p65; Santa Cruz Biotechnology).
The amount of primer and polymerase required for the binding reaction was determined using the SMRT bell concentration (ng/μl) and insert size previously determined using the manufacturer-provided calculator.
Similar(57)
The binding parameters for the reaction were determined using the Stern-Volmer equation.
However, in more complex in vivo systems, the direction of the enzymatic reaction is determined by enzyme specificity for binding either NAD(H) or NADP(H).
For supershift assays, binding reactions were set as described above.
Binding reactions were set up with varying protein combinations.
Anaphylactic reactions were determined.
All reactions were determined in duplicate.
The binding reaction was stopped by washing 5 times with 150 μl of binding buffer.
Total and nonspecific radioligand binding was determined by reaction without and with 10 μM thioperamide and clobenpropit, respectively.
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