As a negative control, PS/PC liposome sedimentation assays were also conducted with the maltose binding protein, which does not bind liposomes.
PRAS40 was identified as a raptor-binding protein, which does not interact with mTORC2.
The negative control would be a protein that does not create microtubule bundles (for example, kinesin-1 motor domain, EB1 or another microtubule-binding protein which does not function as a microtubule-bundling factor in cells).
A benchmark test on a dataset of 525 DNA-binding proteins and 550 proteins which do not bind to DNA using jackknife validation showed that SVM-PSSM-DT achieved the best predicting performance with ACC of 79.96%, MCC of 0.62 and AUC of 86.50%, and performed better than other state-of-the-art methods by 4.56-7.414.56-7.41% of ACC, 5-10.4% in terms of AUC and 0.12-0.18 in terms of MCC.
In human germ cells, Alus (the dominant human SINE) are protected from methylation by the binding of a sperm protein (which does not happen in somatic tissue) [44].
The researchers focused on a class of bacterial proteins called periplasmic binding proteins, which have the unusual characteristic of closing up, like a Venus flytrap, when they bind to a particular chemical.
By devising φc analysis, which is analogous to conventional φ analysis, we have determined the structure of the cleavable form of Escherichia coli maltose-binding protein (MBP), which does not accumulate any partially cleaved products.
Endoglin, a TGF-beta binding protein which maps to chromosome 9q3, is the gene for HHT1.
The v-myb oncogene and its cellular homolog c-myb encode sequence-specific DNA-binding proteins which regulate transcription from promoters containing Myb-binding sites in animal cells.
This Gram-positive bacterium possesses specific surface proteins such as fibronectin-binding proteins, collagen-binding proteins, and fibrinogen-binding proteins, which have been implicated as mediators in specific bacterial binding to the extracellular matrix and subsequent biofilm development [1, 5 7].
