Sentence examples for binding proteins were then from inspiring English sources

ATP binding proteins (ABP) were further purified by incubating the fusions with ATP agarose beads, proteolyzing the bound fusions with thrombin, washing away the MBP with buffer, and eluting the free ABP with phosphate buffer supplemented with 10 mM ATP. ATP binding proteins were then dialyzed with phosphate buffer to remove the excess ATP ligand.

The associated binding proteins were then identified by mass spectrometry.

Captured mRNA binding proteins were then resolved by 3%88% Novex Tris-Acetate gels (Life Technologies SDS-PAGEE and analyzed by western blotting.

Liberated mRNA binding proteins were then precipitated by adding an equal volume of 20% TCA, incubating on ice for 20 min and pelleted in a refrigerated microcentrifuge for 20 min at 13,000 rpm.

IgE-binding proteins were then detected using biotinylated goat antihuman IgE antibody (Kirkegaard and Perry Laboratories, UK) followed by incubation with streptavidin-conjugated alkaline phosphatase (Bio-Rad) for 30 minutes at room temperature.

Additional DNA-binding, linker and MT-binding proteins are then recruited to CEN-bound CBF3.

The desired drug-binding proteins are then purified in a batchwise manner.

OCSs, TRs, RRs and other non-SF DNA-binding proteins are then divided into 76 families depending upon which domains are present in the proteins.

The column was washed with binding buffer, and proteins were then eluted with 10%, 60%, and 100% elution buffer, containing 20 mM potassium phosphate buffer, 500 mM NaCl, and 500 mM imidazole (pH 7.4).

The VEGF moieties of the fusion proteins were then examined for binding to heparin.

These mutant proteins were then tested for binding to fluorescein-labeled CP2 and CP4 by FA.