Sentence examples for binding proteins were removed from inspiring English sources

After the binding to TALON metal affinity resins (Takara), non-specific binding proteins were removed by washing with a buffer containing 50 mM Na2HPO4, 0.3 M NaCl, and 30 mM imidazole (pH 7.0).

Simultaneously, non-specifically binding proteins were removed from kidney tissue lysates by pre-clearing.

Nonspecific binding proteins were removed by washing in cold washing buffer containing 100 mM NaF, 50 mM HEPES (pH 7.5) and 150 mM NaCl for four times followed by one more wash in PBS.

When these binding proteins are removed by proteolysis, TGFβ is activated.

The CF-CM from which heparin-binding proteins were removed, had a significantly enhanced stimulatory effect on DNA synthesis in HUVEC compared to that of 'whole CF-CM'whole

Low-strength, metal-binding proteins were removed by washing with buffer-A containing 50 mM imidazole.

The maltose binding protein was removed after cleavage over night at 4°C with Factor XA (1 unit of Factor XA per 50 µg of fusion protein) and separated from the LDH by gel filtration on a Sephacryl S-300 column.

Washing was performed with the lysis buffer four times after binding, and the proteins were removed from the resin in SDS sample buffer.

After initial protein binding with 1 mM imidazole, weakly bound proteins were removed using sequential washes with 5 mM imidazole (2×), 10 mM imidazole (2×), and 20 mM imidazole (2×).

Unbound proteins were removed by washing twice with 10 ml GST binding buffer, followed by two washes of 10 ml of GST binding buffer containing 1% Triton × 100 to remove unspecific bound proteins.

The unbound excess E. coli proteins were removed from the column by washing it with 25 ml binding Buffer.