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Liposome binding proteins were recovered after centrifugation from the top fraction.
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The bound proteins were recovered by binding to 25 µL of protein-A agarose (Sigma).
The bound proteins were recovered by binding to 25 μl of protein-A agarose (Sigma).
The bound proteins were recovered by binding to 25 μl of protein-A agarose (Sigma, St . Louis MO, USA).
Then, the proteins were recovered from the GST-tag by thrombin cleavage and thrombin was removed by binding to benzamidine-Sepharose.
Proteins were recovered directly from the gels.
Proteins were recovered and 1 50 trypsin was added.
Proteins were recovered in Laemmli buffer and analyzed by immunoblot.
One motif, CHxC occurring in several proteins was recovered.
However, the binding ability of immune sera to the METH-modified target protein was recovered 34 days after meth-free clearance time.
Total protein was recovered after sonication and centrifugation.
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