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To determine plasma protein binding, proteins were precipitated with 10% sulphosalicylic acid, and the radioactivity in protein precipitate and supernatant was measured.
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The Protein G agarose beads binding with antibodies and their associated proteins were precipitated and washed extensively with the lysis buffer five times.
Proteins were precipitated using dichloromethane and methanol.
GFP-tagged proteins were precipitated using GFP-Trap®_A beads (Chromotek), while FLAG-tagged proteins were precipitated using ANTI-FLAG® M2 Affinity Agarose Gel (Sigma).
Proteins were precipitated by adding 2 mL pre-chilled 7.5 M ammonium acetate.
Whole saliva and iSG-derived saliva proteins were precipitated with acetone.
The proteins were precipitated with cooled acetone and lyophilized.
Briefly, proteins were precipitated by chloroform/methanol.
Proteins were precipitated as for SDS-PAGE analysis.
Proteins were precipitated on ice for 10 min and centrifuged.
Biotin-labeled proteins were precipitated using the streptavidin-agarose beads.
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