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Exact(7)
After washing with binding buffer (PIERCE), the binding proteins were eluted with 0.1 M glycine-HCl (pH 2.2).
The specific Tpm1 binding proteins were eluted using different buffers: 0.5 M KCl or 3 M urea in HEK buffer.
AAV-ITR binding proteins were eluted out, dialyzed overnight following the instruction of the kit, and subjected to western blot analysis.
Binding proteins were eluted with a salt gradient of 0 - 1 M NaCl.
The non-specific binding proteins were eluted with 10 CV of PBS buffer.
The beads were then proper washed and the binding proteins were eluted by 1× SDS-PAGE sample loading dye.
Similar(53)
Finally the tightly binding proteins are eluted with excess free drug or under highly denaturing conditions.
Choline-binding proteins were eluted from S. pneumoniae as described above.
Binding protein was eluted by the same buffer, the eluted protein was pooled and loaded on an anion-exchange Mono Q column which was equilibrated with 20 mM Tris HCl buffer (pH 8.2).
The drug-binding proteins are eluted from the affinity beads with a high salt solution or a detergent containing solution following brief centrifugation.
After washing with binding buffer, proteins were eluted with 50 mM glycine/HCl pH 2.8 and immediately neutralized with 0.5 M Tris.
More suggestions(20)
interacting proteins were eluted
binding proteins were precipitated
binding proteins were recovered
binding partners were eluted
binding proteins were excluded
binding RNAs were eluted
binding proteins were analyzed
binding proteins were purified
binding proteins were observed
binding sequences were eluted
binding proteins were found
binding proteins were quantified
binding signals were eluted
binding proteins were studied
binding proteins was eluted
binding proteins were incubated
binding proteins were fitted
binding proteins were determined
binding proteins were deduced
binding proteins were collected
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