Sentence examples for binding proteins using a from inspiring English sources

In order to further assess the role of MAD2B in cell cycle control, we set out to identify binding proteins using a yeast two-hybrid interaction trap (see Materials and methods).

As an initial step to define molecular mechanism of TB10-dependent apoptotic process in ovarian tumor cells, we searched a human ovary cDNA library for a novel TB10 binding protein using a yeast two-hybrid system.

Shortly after the identification of ASK1, ASK2 (also designated as MAP3K6) was identified as an ASK1 binding protein using a yeast two-hybrid screen [ 37].

When we screened the Ci-binding proteins using a yeast two-hybrid assay, we have also identified importin α3, in addition to Rdx (see Materials and methods).

AATYK2/cprk was isolated as a p35-binding protein using a yeast two-hybrid system [5], as was the case for AATYK1 [15].

Subsequently, we confirmed properties of this protein as an I2 binding protein using biochemical and pharmacological techniques.

A method developed by Liu et al. [ 6] predicts RNA-binding sites in proteins using a random forest.

We established a high-yield purification protocol for recombinant prokaryotic expression of wild-type and variant MCAD proteins using a maltose binding protein (MBP -tag.

Previous reports have demonstrated small molecule binding to protein using a high density sensor surface and suggest that aptamer immobilization at higher densities may support a more sensitive and stable method for measuring small molecule aptamer binding.

A recent report characterizing EGFR as a DNA-binding protein using unbiased approaches [ 95] further supports the notion that the nuclear EGFR family plays a role in transcriptional regulation.

Using RNA polymerase as a model system, our laboratory has shown that it is possible to measure the apparent (or effective) target size of a DNA-binding protein using DNA curtains to visualize how RNAP searches for promoters in real time.