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In this case, it would not be difficult to find the corresponding poly(A) binding proteins that could serve as the linker molecules.
To detach Aβ42 from cellular binding proteins that could occlude specific epitopes, cell extracts (200 μL) were mixed with 500 μL of 70% formic acid and sonicated.
However it is broadly expressed and belongs to a bigger family of RNA binding proteins, that could possibly perform overlapping functions.
It is possible that fusing GFP to the amino terminus of Cx43 may be blocking Cx43 binding proteins that could be required for efficient tumor cell diapedesis, although, to date, no amino-terminal Cx43 binding proteins have been identified.
More than 80% of the annotated genes could be grouped into nine categories: amino-acid, carbohydrate and lipid metabolism, biotic or abiotic stress response, terpenoid biosynthesis, transcription/translation processes, transport, and two groups containing generic P450 and protein/DNA- binding proteins that could not be further classified due to lack of more specific information.
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I went on to choose a Ph.D. project with an industrial angle, working at Southampton University on a patented antibody binding protein that could have important medical applications.
We previously reported the isolation of ATXR5 and ATXR6, two PCNA-binding proteins that could be involved in the regulation of cell cycle or cell death.
Nogales and Wang proposed that the ribbon structure (and the sheet structure in general) could provide a novel surface for microtubule-binding proteins that could recognize surfaces unique to the sheet bond to track microtubule growing ends [5].
The S6 protein regulates the translation of ribosomal protein, elongation factor, and polyA-binding protein, that could lead to ribosome biogenesis [ 81- 83].
One of these proteins was identified by MS as the T. vaginalisα-actinin3 (TvandN3) and characterized as an RNA-binding protein that could be involved in the iron posttranscriptional regulation in T. vaginalis.
In summary, our results showed that TvACTN3 is one of the cytoplasmic proteins acting as an RNA-binding protein that could participate in iron regulation at the posttranscriptional level by a mechanism mediated by RNA-protein interactions parallel to the IRE/IRP system.
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