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IGF-2 was measured by RIA [46] after extraction of IGF binding proteins on a BioGel P-10 (Bio-Rad, Hercules, CA) column equilibrated in 1 M acetic acid.
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The LEU3 gene, containing a mutation that confers constitutive activation activity on Leu3, was fused to the gene for maltose binding protein on a URA3-containing 2 micron-based plasmid, and transformed into BY4720-leu3Δneo.
In its original design, the affinity/HPLC system for assessment of folate form distribution is based on the use of purified and immobilized high-affinity folate binding protein on a matrix to concentrate and purify folate from biological samples.
This enabled the quantitative measurement of competitive binding between two RNA-binding proteins on a transcriptome-wide scale.
(B ) A proof-of-principle assay was conducted using known methylated DNA-binding proteins on a pilot protein microarray.
A chemical cross linking experiment using iodinated TGF-β1 and -β5, revealed four specific binding proteins on XTC cells.
Finally the expression of CPMV binding proteins on APCs was evaluated by flow cytometry.
A two-fold increase in the binding of HAGG to activated platelets was obtained, confirming that C1q acts as an immunoglobulin binding protein on platelets.
ANXA2 has been shown to be expressed as a vitamin D binding protein on hepatocytes and HSCs.
They are engineered binding proteins based on a scaffold of the three-α-helix bundle Z-domain (Friedman and Ståhl [2009]; Nilsson and Tolmachev [2007]; Nygren [2008]).
10.7554/eLife.00049.004 Figure 2. Identification of pre-S1 binding protein on primary hepatocytes with photoreactive peptide Myr-47/WTb.
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