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Binding proteins can limit the transport of a peptide to the interstitial space and access to its specific receptors, modulate its biological activity, as well as protecting it from metabolic clearance by proteases and thereby prolonging the half-life in the circulation.
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These data indicate that enhancer-binding proteins can limit gene expression and are not necessarily co-activators.
Whereas RNAi is generally limited to inhibiting target expression, artificial DNA binding proteins can be targeted to virtually any sequence, and can be fused to either transcription repression or activation domains 53, 54.
The sequence specificities of RNA-binding proteins display deep evolutionary conservation, and the recognition preferences for a large fraction of metazoan RNA-binding proteins can thus be inferred from their RNA-binding domain sequence.
These responses can be further modulated by the IGF-binding proteins (IGFBPs) and the IGFBP-related proteins (IGFBP-rPs), which can limit binding of ligand to receptor or elicit their own cellular signals.
Protein energy malnutrition can limit functional performance.
Andrews, N. C. & Faller, D. V. A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells.
Heterochromatin binding protein HP1 can bind with H3K9me3 [13].
The binding of free transition metals to proteins can control their reactivity and limit their availability for the Fenton reaction [30, 32].
The binding of free transition metals to proteins can control their reactivity and limit their availability for the Fenton reaction [ 30, 32].
Both binding and folding of proteins can affect the binding free energy between two proteins.
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