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Such one-directional changes in shape of the actin filaments presumably help to regulate how other actin binding proteins can interact with the filament and consequently regulate the roles of the filaments themselves.
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To test the possibility that Sam68 could affect U2AF binding by protein-protein interaction, we initially tested whether the two proteins can interact in vitro.
These results suggest that Ssa proteins can interact with the NPC through binding to certain Nups, such as Nup116p.
The basic region is a DNA-binding domain, and the amphipathic α-helices of two bHLH proteins can interact with each other.
As suggested by the reviewers, we tested whether Ssa proteins can interact with nucleoporins, and allow tRNAs to interact with nucleoporins by solution binding assays.
Hsp40 proteins can interact with the hydrophobic side chains of non-native polypeptides through the peptide-binding fragment and prevent the polypeptides from aggregating (4, 5).
To determine if Sp1 or other proteins can interact with the vesnarinone-responsive element, electrophoretic mobility-shift assay was performed using the oligonucleotides containing the Sp1-binding sites.
Also, heat-denatured whey proteins can interact with MFGM proteins.
The MYB4 protein can interact with its own MYB binding motif, thus regulating its own expression through an autoregulatory mechanism.
Here, we show that the RUNX1 protein can interact with an AF4-MLL complex and stabilize its binding to certain gene targets.
These include the transcription factor CCAAT/enhancer-binding protein, which can interact with CREB, and a dopamine receptor (Alberini, 2005; El-Ghundi et al., 2007).
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