An extreme example was the synthesis of the ligand for the vitamin D binding protein, which required 23 steps, and a synthesis to be attempted only by highly experienced synthetic organic chemists [ 50].
Structure-based approaches, like DOCK [3, 4] or PLANTS [5], try to dock the ligand into the binding pocket of a target protein, which requires X-ray diffraction or nuclear magnetic resonance spectroscopy experiments to obtain three-dimensional coordinates of the protein structure.
The removal of N-linked oligosaccharides could disturb the interaction between hTfR2 and a carbohydrate binding protein, which is required for conveying hTfR2 from a degradative to a recycling pathway after holo-Tf treatment.
The method uses sepharose as the matrix for the immobilized folate binding protein (21), which requires manual injection of the sample into the HPLC system.
The three far-upstream element (FUSE) binding proteins (FBP1, FBP2, and FBP3) belong to an ancient family of single-stranded DNA binding proteins which are required for proper regulation of the c-myc proto-oncogene.
At low oxygen concentrations the carboxyl-terminal transactivation domain of HIF-1α recruits several co-activators, including p300 and CREB-binding protein, which are required for HIF-1 signalling [ 35, 36].
Indeed, this is true of TBP, the so-called TATA-binding protein, which is required for the assembly of the preinitiation complex at all loci even though only 10 24% of human promoters have a TATA box [ 73, 74].
Furthermore, the aphVIII gene encodes an enzyme and not simply a binding protein like Ble, which requires a 1 1 ratio of expressed protein and antibiotic molecules.
In particular, the amino-terminal domain of CEACAM1 is necessary, but not sufficient for Opa protein-independent binding, which requires multiple extracellular domains of the human receptor in a cellular context.
EPLIN is an actin-binding protein, which functions to bundle actin filaments; therefore, EPLIN presence is required at AJ along with filamentous actin.
