PRAS40 was identified as a raptor-binding protein, which does not interact with mTORC2.
The negative control would be a protein that does not create microtubule bundles (for example, kinesin-1 motor domain, EB1 or another microtubule-binding protein which does not function as a microtubule-bundling factor in cells).
A related class of cupins is the auxin binding proteins, which do not show catalytic activity but work as signal transducers in plant cells.
In human germ cells, Alus (the dominant human SINE) are protected from methylation by the binding of a sperm protein (which does not happen in somatic tissue) [44].
This type of binding can be applied neither to Alzheimer's Aβ-peptides nor to Parkinson's AS protein, which do not contain Cys residues.
A benchmark test on a dataset of 525 DNA-binding proteins and 550 proteins which do not bind to DNA using jackknife validation showed that SVM-PSSM-DT achieved the best predicting performance with ACC of 79.96%, MCC of 0.62 and AUC of 86.50%, and performed better than other state-of-the-art methods by 4.56-7.414.56-7.41% of ACC, 5-10.4% in terms of AUC and 0.12-0.18 in terms of MCC.
The concept of heteromultivalency is also increasingly used in the design of high affinity ligands toward proteins which do not have two similar binding sites.
By devising φc analysis, which is analogous to conventional φ analysis, we have determined the structure of the cleavable form of Escherichia coli maltose-binding protein (MBP), which does not accumulate any partially cleaved products.
The researchers focused on a class of bacterial proteins called periplasmic binding proteins, which have the unusual characteristic of closing up, like a Venus flytrap, when they bind to a particular chemical.
Additional support for the importance of this cellular organization pattern for PR expression to normal mammary gland development comes from the recent analysis of the adult CCAAT/enhancer binding protein β knockout mammary gland, which does not respond to the proliferative effects of estrogen and progesterone [ 23].
