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Recombinant full-length human Bcl-xL was cloned into PTYB1 vector to produce a fusion of Bcl-xL with an intein/chitin binding protein, which can be removed to obtain Bcl-xL with vector encoded residues Gly Ser Ser at the C terminus.
Classically, the cytotoxic compound is conjugated to the targeting mAb via nondirected chemical linkage, often resulting in an undefined stoichiometry of ADCs and heterogenic positioning of the cytotoxic payload in relation to the binding protein, which can directly affect its therapeutic efficacy (Ducry and Stump, 2010).
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One focuses on odorant binding proteins, which can be classified into several functional groups.
The method eliminates most of the binding proteins, which can interfere with the RIA.
Tbx proteins are sequence-specific DNA binding proteins which can serve as transcriptional repressors or activators (Wardle and Papaioannou, 2008).
A further relation to muscle function can be found in the histidine-rich Ca2+-binding protein which can also bind Zn and is localized in the sarcoplasmic reticulum [ 135].
GRAIN LENGTH7/GRAIN WIDTH7 (GL7/GW7) encodes a putative microtubule-binding protein which can regulate gain length and grain size diversity (Wang et al., 2015a; Wang et al., 2010).
Cdc42 is a small GTP-binding protein which can regulate the formation of actin filament-based structures.
These include the transcription factor CCAAT/enhancer-binding protein, which can interact with CREB, and a dopamine receptor (Alberini, 2005; El-Ghundi et al., 2007).
Calmodulin (CaM) is a ubiquitous Ca2+-binding protein, which can regulate a number of different protein targets, thereby affecting many different cellular functions (Chin and Means 2000).
The name, regucalcin, was proposed for this calcium-binding protein, which can regulate various Ca2+-dependent enzymes activation in liver cells.
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