The general amino acid ABC-transporter protein for AapJ (substrate binding protein) was detected by proteome analysis: the aapJ gene is part of the aapJQMP operon that exists in many Rhizobiaceas and has been described in detail in R. leguminosarum [ 37].
The specific binding protein was detected with biotinylated anti-PD-1 mAb (bio-9H1, 1 μg/ml) [ 31, 34] for 1 h at 37 °C, followed by streptavidin-horseradish peroxidase at 1 2000 for 1 h at 37 °C, and then revealed with the substrate 3,3′,5,5′-tetramethylbenzidine 3,3′,5,5′-tetramethylbenzidine USA).
Binding sites for CCAAT/enhancer binding proteins were detected in all alleles of the pmt locus.
While cytokine and chemokine receptors and binding proteins were detected, the ligand factors were below detectable levels, as expected.
Calmodulin was measured by radioimmunoassay and the binding proteins were detected by interaction of 125I-calmodulin with matrix proteins distributed on one-dimensional polyacrylamide gels.
Numerous DNA binding proteins were detected from each of the Cu II) grown cultures; including DNA binding ferritin Dps family proteins (Krad1159,4280) which possess a ferroxidase center for metal sequestration and has been shown to protect DNA against oxidative damage [53].
No other retinoid binding proteins were detected in the peak.
The binding proteins were detected by using enhanced chemiluminescence using LuminataTM Forte (Millipore, Bedford, USA).
Several RII binding proteins were detected in INS-1 832/13 INS-1 832/13cells by overlay assay.
In this study, 21 DEGs encoding Ca2+ binding proteins were detected in the C. komarovii transcriptome.
