Sentence examples for binding protein using the from inspiring English sources

Antiserum was produced against a fragment of Arabidopsis CAO that had been fused to the maltose binding protein using the pMAL fusion system (New England Biolabs, Beverly, MA).

We have evaluated the conservation of residues in each RNA binding protein using the server, Consurf [ 24] available at http://consurf.tau.ac.il/.

In addition, we have analysed the energetic and electrostatic properties of ubiquitin-like domains in complex with various functional binding proteins using the protein design algorithm FoldX.

Since HMG boxes contain three helices (see above), I predicted the secondary structures of N- and C-terminal regions of B-block binding proteins using the PSIPRED method [ 29].

The AP-2 and Ets sites have been targeted by either preventing the binding of the cognate binding protein (using sodium aurothiomalate for AP-2 [ 16] and DNA-binding polyamides for Ets factors [ 17]) or by creating fusion proteins between the appropriate DNA-binding domain and a transcriptional repressor [ 4], in all cases impairing promoter activity in functional assays.

Various assays for dipeptide transport by the dipeptide and tripeptide permeases of Escherichia coli were performed, together with measurements of thermodynamic parameters of substrate binding to the dipeptide binding protein using isothermal titration calorimetry.

Moreover, these TFs may interact with different binding proteins using different regions of the homeodomain depending on their dimerizing partners, which will then also affect the spacing between the two proteins [ 45].

Levels of radioactivity in the I-microscales were converted to total and nonspecific binding (fmol mg−1 protein) using the specific activity of the I-microscales supplied by the manufacturer (Amersham).

Aliquots of the fractions that were coloured by anisaldehyde on thin-layer plates were tested for binding of the CS6 protein using the chromatogram binding assay.

Finally, we describe a method for visualizing dynamic assembly and disassembly of single actin filaments and fluorescently labeled actin binding proteins using total internal reflection fluorescence (TIRF) microscopy.

A recent report characterizing EGFR as a DNA-binding protein using unbiased approaches [ 95] further supports the notion that the nuclear EGFR family plays a role in transcriptional regulation.