An RV xF motif, common to many PP1 binding proteins, is present in the conserved functional core of the PPP1R15 family members and their viral counterparts, and plays an important role in assembly of the active holophosphatase complex (He et al., 1998; Connor et al., 2001; Novoa et al., 2001).
A locus coding for the periplasmic ferric iron binding protein FbpA, permease FbpB and a putative iron-transport system ATP-binding protein is present (LHK_02634-02636).
Again, we thank the reviewer for this excellent suggestion and agree that experiments with a low density of NEM-myosin on the surface while another side-binding protein is present in solution would help in clarify the origin of pauses.
A remnant of a β-like phage was detected at this genomic position in the genome sequence of C. ulcerans 809, and the rbp gene encoding a ribosome-binding protein is present in the immediate vicinity.
Moreover, the S. cerevisiae MAT loci code for only three proteins (the homeodomain proteins a1 and α2 and the "α-domain" protein α1), whereas an additional gene (MAT a2) coding for an HMG domain DNA-binding protein is present in the MAT a idiomorph of several species (Butler et al. 2004).
Given that many protein domains have sequence lengths between 50 and 100 amino acids and ATP binding proteins are present in every major enzyme class, we felt that the random region and target choice were appropriate to identify small protein domains with simple ligand binding activity [6].
Regarding the pattern recognition receptors, a great variety of PGRPs (peptidoglycan recognition receptors) and GNBPs (gram negative binding proteins) are present in this tissue.
Intriguingly, chromatin regulators encompassing histone modifiers and histone modification binding proteins are present in a combinatorial fashion at distinct genomic loci and frequently bring together regulators associated with opposing activities [27].
In the case of yku70Δ cells bearing telomere repeat tracts that are ~200 bp shorter than those of wild-type cells, we hypothesized that this resection may reduce these tracts to the point where so few telomere binding proteins are present that the telomere becomes dysfunctional and Mec1-HAp is recruited.
