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Calcium-Response Factor (CaRF) is a novel nuclear protein first identified as a binding protein for a calcium-response element (CaRE1) in Bdnf promoter IV [14].
Nucleolin expressed at the cell surface is a binding protein for a variety of ligands implicated in tumorigenesis and angiogenesis.
Cell surface expressed gC1q-R can serve as a receptor for diverse proinflammatory ligands, and as a binding protein for a number of functional antigens of viral and bacterial origin.
Instances found in genome annotations include orthologues of the S. pneumoniae TIGR4 CDS SP_0243, encoding the extracellular binding protein for a putative iron ABC transporter, which is disrupted by the insertion of a RUP element in all the other pneumococcal genomes except S. pneumoniae AP200, 670-6B and TIGR4 itself.
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We have shown that highly stable binding proteins for a wide spectrum of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus.
Two principally different applications are considered, (1) effects monitoring with biological components as targets for bioeffective substances, among them endocrine disruptors; and (2) immunochemical analysis employing antibodies as binding proteins for a wide variety of analytes such as pesticides.
Binding proteins for a diverse set of model targets could be isolated from this library; our chosen targets included a small organic molecule (fluorescein), a 12 amino acid peptide fragment from the C-terminus of β-catenin, the model proteins hen egg lysozyme and streptavidin, and immunoglobulins from chicken and mouse.
Reverse docking approach, the opposite of the direct docking method firstly proposed by Chen et al. [ 11], could identify probable binding proteins for a specific small molecule.
We found that caveolin-1 is a novel binding protein for Mdm2, a negative regulator of p53.
However, in many cases it is desirable to profile targets of an RNA binding protein for which an immunoprecipitation-grade antibody is lacking.
Among them, CD74 encodes a Type II transmembrane protein, which is a binding protein for MIF and an essential protein for MIF-induced activation of extracellular signal-regulated kinase-1/2MAP kinase cascade, cell proliferation and apoptosis [ 38].
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